Investigation of Growth Factors and Mathematical Modeling of Nutrient Media for the Shoots Multiplication In Vitro of Rare Plants of the Rostov Region

Micropropagation is an effective way to preserve the gene pool of threatened plants. This study devoted mathematical modeling nutrient media and effect mT (meta-topoline) on multiplication shoots Hedysarum grandiflorum, Hyssopus cretaceus, Matthiola fragrans in vitro comparison with benzylaminopurine (BAP) kinetin (KT). Initiation was performed MS medium 0.5 mg/L BAP. For multiplication, MS, B5, WPM were used addition mT, BAP, KT. H. coefficient highest B5 at a concentration 1 mg/L—2.90 per plant, for cretaceus—B5 + M. fragrans—B5 A positive grandiflorum found. The efficiency using KT cretaceus shoot shown. effectiveness determined. micropropagation has been demonstrated. It not recommended use these plant species.